Mouse OVA sIgE (Ovalbumin Specific IgE) ELISA Kit
Mouse OVA sIgE (Ovalbumin Specific IgE) ELISA Kit
The Mouse OVA sIgE (Ovalbumin Specific IgE) ELISA Kit measures OVA sIgE in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on capture enzyme-linked immune-sorbent assay technology. Anti Mouse IgE Antibody was pre-coated onto the 96-well plate. The HRP conjugated antigen was used as the detection antigen. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, HRP detection antigen was added to bind with OVA sIgE conjugated on Anti Mouse IgE Antibody. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of OVA sIgE in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.
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