Mouse OVA sIgM (Ovalbumin Specific IgM) ELISA Kit
Mouse OVA sIgM (Ovalbumin Specific IgM) ELISA Kit
The Mouse OVA sIgM (Ovalbumin Specific IgM) ELISA Kit measures OVA sIgM in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on capture ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti Mouse IgM(U-chain). HRP-antigen is used as detection antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-antigen. Then, it binds with OVA sIgM bound to anti Mouse IgM(U-chain). Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of OVA sIgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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