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Mouse PADI4 (Protein-arginine Deiminase Type-4) ELISA Kit
Mouse PADI4 (Protein-arginine Deiminase Type-4) ELISA Kit
The Mouse (PADI4) Protein-arginine Deiminase Type-4 ELISA Kit measures Protein-arginine deiminase type-4 in Mouse samples. The plate has been pre-coated with Mouse PADI4 antibody. PADI4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PADI4 Antibody is added and binds to PADI4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PADI4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PADI4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1640Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.08 ng/mL
Detection Range
0.2-60 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:32528174).
Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660).
Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:24463520, PubMed:32528174).
Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:20733033, PubMed:23650392).
Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:20733033, PubMed:32528174).
Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (By similarity).
Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Protein-arginine deiminase type-4
Regulatory Status
For Research Use Only
