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Mouse PI (Proinsulin) ELISA Kit
Mouse PI (Proinsulin) ELISA Kit
The Mouse (PI) Proinsulin ELISA Kit measures Proinsulin in Mouse samples. The plate has been pre-coated with Mouse PI antibody. PI present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PI Antibody is added and binds to PI in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PI antibody. After incubation unbound Streptavidin- HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PI. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0390Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.56 ng/L
Detection Range
1-400 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Cancer, Immunology, Diabetes Research, Endocrinology, Metabolism
Background
Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28073925, PubMed:8496151, PubMed:28541286, PubMed:28453791, PubMed:20026663, PubMed:23041626, PubMed:27789271, PubMed:12524533).
Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286, PubMed:27789271).
Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925).
In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (PubMed:12524533).
Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:8496151).
Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed:23041626, PubMed:20026663).
Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.
Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Proinsulin
Regulatory Status
For Research Use Only
