The Mouse (PLA2G2F) Group 2F Secretory Phospholipase A2 ELISA Kit measures Group 2F secretory phospholipase A2 in Mouse samples. The plate has been pre-coated with Mouse PLA2G2F antibody. PLA2G2F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PLA2G2F Antibody is added and binds to PLA2G2F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G2F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PLA2G2F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundSecretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10531313).
Hydrolyzes the ester bond of the fatty acyl group attached at the sn-2 position of phospholipids (phospholipase A2 activity), the catalytic efficiency decreasing in the following order: phosphatidylglycerols > phosphatidylethanolamines > phosphatidylcholines > phosphatidylserines (PubMed:11877435, PubMed:10531313).
May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases (PubMed:10531313). Source: UniProt Consortium (2025)
