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Mouse PLA2R1 (Secretory Phospholipase A2 Receptor) ELISA Kit
Mouse PLA2R1 (Secretory Phospholipase A2 Receptor) ELISA Kit
The Mouse (PLA2R1) Secretory Phospholipase A2 Receptor ELISA Kit measures Secretory Phospholipase A2 Receptor in Mouse samples. The plate has been pre-coated with Mouse PLA2R1 antibody. PLA2R1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PLA2R1 Antibody is added and binds to PLA2R1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLA2R1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PLA2R1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2444Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.024 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipases sPLA2-IB/PLA2G1B, sPLA2-X/PLA2G10 and, with lower affinity, sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B and sPLA2-X/PLA2G10. In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Secretory Phospholipase A2 Receptor
Regulatory Status
For Research Use Only
