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Mouse POGLUT1 (Protein O-Glucosyltransferase 1) ELISA Kit

Mouse POGLUT1 (Protein O-Glucosyltransferase 1) ELISA Kit

The Mouse (POGLUT1) Protein O-Glucosyltransferase 1 ELISA Kit measures Protein O-Glucosyltransferase 1 in Mouse samples. The plate has been pre-coated with Mouse POGLUT1 antibody. POGLUT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse POGLUT1 Antibody is added and binds to POGLUT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated POGLUT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse POGLUT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0720Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.151 ng/mL
Detection Range 0.3-90 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed: 21949356, PubMed: 26496195). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed: 21949356, PubMed: 26496195). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (By similarity). Notch glucosylation does not affect Notch ligand binding (By similarity). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (PubMed: 26496195). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Protein O-Glucosyltransferase 1
Regulatory Status For Research Use Only
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