The Mouse (PPIF) Peptidyl-Prolyl Cis-Trans Isomerase F, Mitochondrial ELISA Kit measures Peptidyl-prolyl cis-trans isomerase F, mitochondrial in Mouse samples. The plate has been pre-coated with Mouse PPIF antibody. PPIF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PPIF Antibody is added and binds to PPIF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPIF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PPIF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (By similarity).
Involved in regulation of the mitochondrial permeability transition pore (mPTP) (PubMed:15800626, PubMed:15800627, PubMed:16103352, PubMed:18684715, PubMed:31489369).
It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated (PubMed:15800626, PubMed:15800627, PubMed:16103352, PubMed:18684715, PubMed:31489369).
In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis (PubMed:22726440).
Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels (PubMed:19801635, PubMed:21281446).
Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (By similarity). Source: UniProt Consortium (2025)
