This ELISA kit is designed to detect Mouse Pulmonary Surfactant-Associated Protein D (Mouse SFTPD). The assay plate has been pre-coated with mouse anti-Mouse Surfactant protein D monoclonal antibody. When the sample containing Surfactant protein D is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse Surfactant protein D Antibody is added to the wells and binds to Surfactant protein D in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse Surfactant protein D present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Research AreasCancer, Cell Biology, Immunology, Metabolism, Microbiology, Signal Transduction
BackgroundSurfactant pulmonary-associated protein D, also known as SFTPD and SP-D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. The polymorphic variation in the N-terminal domain of the SP-D molecule influences oligomerization, function, and the concentration of the molecule in serum. SFTPD is produced primarily by alveolar type II cells and nonciliated bronchiolar cells in the lung and is constitutively secreted into the alveoli where it influences surfactant homeostasis, effector cell functions, and host defense. It is upregulated in a variety of inflammatory and infectious conditions including Pneumocystis pneumonia and asthma. SFTPD is humoral molecules of the innate immune system, and is considered a functional candidate in chronic periodontitis. Besides, it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SFTPD. Thus, recombinant SFTPD has been proposed as a therapeutical option for cystic fibrosis, neonatal lung disease and smoking-induced emphysema. Furthermore, SFTPD serum levels can be used as disease activity markers for interstitial lung diseases.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
