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Mouse RIPK1 (Receptor-interacting Serine Threonine-protein Kinase 1) ELISA Kit
Mouse RIPK1 (Receptor-interacting Serine Threonine-protein Kinase 1) ELISA Kit
The Mouse (RIPK1) Receptor-interacting Serine Threonine-protein Kinase 1 ELISA Kit measures Receptor-interacting serine/threonine-protein kinase 1 in Mouse samples. The plate has been pre-coated with Mouse RIPK1 antibody. RIPK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse RIPK1 Antibody is added and binds to RIPK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RIPK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse RIPK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2419Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
4.55 ng/mL
Detection Range
10-2000 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Enzyme & Kinase
Background
Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways (PubMed:24813849, PubMed:24813850, PubMed:24557836, PubMed:27819681, PubMed:28842570, PubMed:31511692, PubMed:31827280, PubMed:31827281).
Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival (PubMed:24813849, PubMed:24813850, PubMed:24557836, PubMed:28842570, PubMed:31519886, PubMed:31519887).
Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway (PubMed:31519886, PubMed:31519887).
Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis (PubMed:28842570, PubMed:27819681, PubMed:27819682, PubMed:29440439, PubMed:30988283, PubMed:31519886, PubMed:31519887).
RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis (PubMed:24813849, PubMed:24813850, PubMed:24557836).
In normal conditions, RIPK1 acts as an inhibitor of RIPK3-dependent necroptosis, a process mediated by RIPK3 component of complex IIb, which catalyzes phosphorylation of MLKL upon induction by ZBP1 (PubMed:24557836, PubMed:27819681, PubMed:27819682, PubMed:31358656).
Inhibits RIPK3-mediated necroptosis via FADD-mediated recruitment of CASP8, which cleaves RIPK1 and limits TNF-induced necroptosis (PubMed:31358656).
Required to inhibit apoptosis and necroptosis during embryonic development: acts by preventing the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8 (PubMed:30867408, PubMed:30185824).
In addition to apoptosis and necroptosis, also involved in inflammatory response by promoting transcriptional production of pro-inflammatory cytokines, such as interleukin-6 (IL6) (PubMed:31827280, PubMed:31827281).
Phosphorylates RIPK3: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (By similarity).
Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (By similarity).
Required for ZBP1-induced NF-kappa-B activation in response to DNA damage (PubMed:12654725, PubMed:19590578). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Receptor-interacting serine/threonine-protein kinase 1
Regulatory Status
For Research Use Only
