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Mouse RSPO3 (R-Spondin-3) ELISA Kit
Mouse RSPO3 (R-Spondin-3) ELISA Kit
The Mouse (RSPO3) R-Spondin-3 ELISA Kit measures R-spondin-3 in Mouse samples. The plate has been pre-coated with Mouse RSPO3 antibody. RSPO3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse RSPO3 Antibody is added and binds to RSPO3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RSPO3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse RSPO3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2436Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.97 ng/L
Detection Range
2-600 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis (PubMed:16543246, PubMed:21693646, PubMed:26766444).
Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway (PubMed:16543246, PubMed:21693646).
Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells (PubMed:26766444, PubMed:16543246, PubMed:21693646).
Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3 (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
R-spondin-3
Regulatory Status
For Research Use Only
