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Mouse SCT (Secretin) ELISA Kit
Mouse SCT (Secretin) ELISA Kit
The Mouse (SCT) Secretin ELISA Kit measures Secretin in Mouse samples. The plate has been pre-coated with Mouse SCT antibody. SCT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SCT Antibody is added and binds to SCT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SCT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SCT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1141Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
3.43 ng/L
Detection Range
7-1500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Hormone involved in different processes, such as regulation of the pH of the duodenal content, food intake and water homeostasis (PubMed: 20578263, PubMed: 20739612, PubMed: 20927047, PubMed: 30449620). Exerts its biological effects by binding to secretin receptor (SCTR), a G-protein coupled receptor expressed in the basolateral domain of several cells (PubMed: 30449620). Acts as a key gastrointestinal hormone by regulating the pH of the duodenal content (PubMed: 20578263). Secreted by S cells of the duodenum in the crypts of Lieberkuehn and regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas (PubMed: 20578263). Production of bicarbonate is essential to neutralize the pH and ensure no damage is done to the small intestine by the gastric acid (PubMed: 20578263). In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation (PubMed: 30449620). Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation (PubMed: 30449620). Also able to stimulate lipolysis in white adipocytes (PubMed: 24273196). Also plays an important role in cellular osmoregulation: released into the systemic circulation in response to hyperosmolality and acts at different levels in the hypothalamus, pituitary and kidney to regulate water homeostasis (PubMed: 20739612). Also plays a role in the central nervous system, possibly by acting as a neuropeptide hormone: required for hippocampal synaptic function and neural progenitor cells maintenance (PubMed: 18534766, PubMed: 21159798). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Secretin
Regulatory Status
For Research Use Only
