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Mouse SEMA3F (Semaphorin-3F) ELISA Kit
Mouse SEMA3F (Semaphorin-3F) ELISA Kit
The Mouse (SEMA3F) Semaphorin-3F ELISA Kit measures Semaphorin-3F in Mouse samples. The plate has been pre-coated with Mouse SEMA3F antibody. SEMA3F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA3F Antibody is added and binds to SEMA3F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA3F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SEMA3F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2451Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.052 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993). Sequence comparisons have grouped the secreted semaphorins into 3 general classes (classes 2, 3 and V), all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Semaphorin-3F
Regulatory Status
For Research Use Only
