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Mouse SEMA4D (Semaphorin-4D) ELISA Kit
Mouse SEMA4D (Semaphorin-4D) ELISA Kit
The Mouse (SEMA4D) Semaphorin-4D ELISA Kit measures Semaphorin-4D in Mouse samples. The plate has been pre-coated with Mouse SEMA4D antibody. SEMA4D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA4D Antibody is added and binds to SEMA4D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SEMA4D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2453Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.056 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (By similarity). Regulates GABAergic synapse development (PubMed: 23699507, PubMed: 29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (PubMed: 23699507, PubMed: 29981480). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (By similarity). Promotes the migration of cerebellar granule cells (PubMed: 17554007). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (By similarity). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Semaphorin-4D
Regulatory Status
For Research Use Only
