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Mouse Semaphorin-4D (SEMA4D) ELISA Kit
Mouse Semaphorin-4D (SEMA4D) ELISA Kit
This ELISA kit is designed to detect Mouse Semaphorin-4D (Mouse SEMA4D). The assay plate has been pre-coated with mouse anti-Mouse Semaphorin 4D monoclonal antibody. When the sample containing Semaphorin 4D is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse Semaphorin 4D Antibody is added to the wells and binds to Semaphorin 4D in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse Semaphorin 4D present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE329
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
10.24 pg/mL
Detection Range
46.88-3000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Immunology, Neuroscience
Background
Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Semaphorin-4D
Regulatory Status
For Research Use Only
