The Mouse (SIRT3) Nad-dependent Protein Deacetylase Sirtuin-3 ELISA Kit measures Nad-Dependent Protein Deacetylase Sirtuin-3 in Mouse samples. The plate has been pre-coated with Mouse SIRT3 antibody. SIRT3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SIRT3 Antibody is added and binds to SIRT3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SIRT3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SIRT3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundNAD-dependent protein deacetylase (PubMed: 23835326, PubMed: 17923681, PubMed: 18794531, PubMed: 21172655, PubMed: 26620563). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed: 23835326, PubMed: 17923681, PubMed: 18794531, PubMed: 21172655). Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP Synthase subunit ATP5PO (PubMed: 16790548, PubMed: 18794531, PubMed: 21172655). Contributes to the regulation of the cellular energy metabolism (PubMed: 23835326). Important for regulating tissue-specific ATP levels (PubMed: 18794531, PubMed: 24252090). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed: 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide Synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (PubMed: 26620563). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteNad-Dependent Protein Deacetylase Sirtuin-3
