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Mouse TSKU (Tsukushin) ELISA Kit

Mouse TSKU (Tsukushin) ELISA Kit

The Mouse (TSKU) Tsukushin ELISA Kit measures Tsukushin in Mouse samples. The plate has been pre-coated with Mouse TSKU antibody. TSKU present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse TSKU Antibody is added and binds to TSKU in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TSKU antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse TSKU. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2530Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.055 ng/mL
Detection Range 0.1-40 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Contributes to various developmental events and other processes such as wound healing and cholesterol homeostasis through its interactions with multiple signaling pathways (PubMed: 21856951, PubMed: 22995554, PubMed: 25159578, PubMed: 31391339). Wnt signaling inhibitor which competes with WNT2B for binding to Wnt receptor FZD4 and represses WNT2B-dependent development of the peripheral eye (PubMed: 21856951). Plays a role in regulating the hair cycle by controlling TGFB1 signaling (PubMed: 22995554). Required for the development of the anterior commissure in the brain by inhibiting neurite outgrowth (PubMed: 21055390, PubMed: 23206892). Essential for terminal differentiation of hippocampal neural stem cells (PubMed: 31983064). Plays a role in regulating bone elongation and bone mass by modulating growth plate chondrocyte function and overall body size (PubMed: 30271858). Required for development of the inner ear through its involvement in stereocilia formation in inner hair cells (PubMed: 32127020). Facilitates wound healing by inhibiting secretion of TGFB1 from macrophages which prevents myofibroblast differentiation, maintaining inflammatory cell quiescence (PubMed: 25159578). Plays a role in cholesterol homeostasis by reducing circulating high-density lipoprotein cholesterol, lowering cholesterol efflux capacity and decreasing cholesterol-to-bile acid conversion in the liver (PubMed: 31391339). In one study, shown to negatively regulate sympathetic innervation in brown fat, leading to reduced energy expenditure (PubMed: 31535079). In another study, shown not to affect brown fat thermogenic capacity, body weight gain or glucose homeostasis (PubMed: 31767170). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Tsukushin
Regulatory Status For Research Use Only
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