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Mouse WNT5B (Protein Wnt-5B) ELISA Kit

Mouse WNT5B (Protein Wnt-5B) ELISA Kit

The Mouse (WNT5B) Protein Wnt-5B ELISA Kit measures Protein Wnt-5b in Mouse samples. The plate has been pre-coated with Mouse WNT5B antibody. WNT5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse WNT5B Antibody is added and binds to WNT5B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT5B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse WNT5B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2402Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.024 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Stem Cells
Background Protein Wnt-5b is a protein that in humans is encoded by the WNT5B gene. The WNT gene family consists of structurally-related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein showing 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates two transcript variants. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Protein Wnt-5b
Regulatory Status For Research Use Only
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