Porcine HMGB1 (High mobility group protein B1) QuickTest ELISA Kit
Porcine HMGB1 (High mobility group protein B1) QuickTest ELISA Kit
The Porcine HMGB1 (High mobility group protein B1) QuickTest ELISA Kit measures HMGB1 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains HMGB1, a capture antibody-HMGB1-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of HMGB1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
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