The Porcine (PPARG) Peroxisome Proliferation Activated Receptor Gamma ELISA Kit measures Peroxisome Proliferation Activated Receptor Gamma in Porcine samples. The plate has been pre-coated with Porcine PPARG antibody. PPARG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine PPARG Antibody is added and binds to PPARG in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPARG antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine PPARG. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundNuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels. Source: UniProt Consortium (2025)
