• Proguanylin ELISA
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Proguanylin ELISA

Proguanylin ELISA

The Proguanylin ELISA is an enzyme immunoassay for the quantitative determination of Proguanylin in human serum and plasma.

The microtiter plate is coated with the antibody specifically binding the Proguanylin. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of Proguanylin in the specimen. The concentration of Proguanylin in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA3007
$560.00 USD
$560.00 USD $560.00 USD
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2 weeks

Product Details

Species Reactivity Human
Sensitivity 5 pg/mL
Detection Range 0.156 - 10 ng/mL
Sample Type Serum, Plasma
Sample Size 10 uL
Incubation(s) 2.5 hour(s)
Research Areas Endocrinology
Background Proguanylin (guanylate cyclase activator 2A, guanylate cyclase-activating protein 1), is a precursor to the hormone guanylin, and plays a significant role in various physiological processes in the kidney and intestine through activation of guanylate cyclase C receptor. In Crohn's disease, serum proguanylin levels are notably reduced compared to healthy individuals, and levels increase following anti-inflammatory treatment, suggesting its potential as a biomarker for disease activity and therapeutic response. In the context of hypertension, elevated proguanylin levels have been observed in individuals with high blood pressure, indicating a possible role in sodium metabolism and blood pressure regulation.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Proguanylin
Regulatory Status For Research Use Only
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