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Rat A2-MG (Alpha2-Microglobulin) ELISA Kit
Rat A2-MG (Alpha2-Microglobulin) ELISA Kit
The Rat (A2-MG) Alpha2-Microglobulin ELISA Kit measures Alpha2-Microglobulin in Rat samples. The plate has been pre-coated with Rat A2M antibody. A2M present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat A2M Antibody is added and binds to A2M in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated A2M antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat A2M. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1365Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
0.24 mg/L
Detection Range
0.5-100 mg/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology
Background
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a Peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Alpha2-Microglobulin
Regulatory Status
For Research Use Only
