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Rat AFM (Afamin) ELISA Kit

Rat AFM (Afamin) ELISA Kit

The Rat (AFM) Afamin ELISA Kit measures Afamin in Rat samples. The plate has been pre-coated with Rat AFM antibody. AFM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AFM Antibody is added and binds to AFM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AFM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat AFM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1261Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.42 ng/mL
Detection Range 1-300 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Functions as carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Afamin
Regulatory Status For Research Use Only
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