1
/
of
1
Rat BETA GD (Beta-glucuronidase) ELISA Kit
Rat BETA GD (Beta-glucuronidase) ELISA Kit
The Rat (BETA GD) Beta-glucuronidase ELISA Kit measures beta-glucuronidase in Rat samples. The plate has been pre-coated with Rat betaGD antibody. betaGD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat betaGD Antibody is added and binds to betaGD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated betaGD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat betaGD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0495Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Rat
Sensitivity
0.02 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Tags and Cell Markers, Signal Transduction
Background
Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human Beta-glucuronidase is a type of glucuronidase (a member of glycosidase Family 2) that catalyzes hydrolysis of Beta-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans) such as heparan sulfate. Human Beta-glucuronidase is located in the lysosome. In the gut, brush border Beta-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption. Beta-glucuronidase is also present in breast milk, which contributes to neonatal jaundice. The protein is encoded by the GUSB gene in humans and by the uidA gene in bacteria. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
beta-glucuronidase
Regulatory Status
For Research Use Only
