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Rat BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit
Rat BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit
The Rat (BMP-4) Bone Morphogenetic Protein 4 ELISA Kit measures Bone morphogenetic protein 4 in Rat samples. The plate has been pre-coated with Rat BMP-4 antibody. BMP-4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat BMP-4 Antibody is added and binds to BMP-4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BMP-4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat BMP-4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0266Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
1.01 ng/L
Detection Range
2-600 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular, Signal Transduction, Cancer, Developmental Biology, Bone Metabolism
Background
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis (By similarity).
Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Bone morphogenetic protein 4
Regulatory Status
For Research Use Only
