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Rat C4 (Complement 4) ELISA Kit
Rat C4 (Complement 4) ELISA Kit
The Rat (C4) Complement 4 ELISA Kit measures Complement 4 in Rat samples. The plate has been pre-coated with Rat C4 antibody. C4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat C4 Antibody is added and binds to C4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated C4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat C4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0160Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
3.42 ng/mL
Detection Range
7-1500 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes (By similarity). Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory processes. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Complement 4
Regulatory Status
For Research Use Only
