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Rat CAPN1 (Calpain 1) ELISA Kit

Rat CAPN1 (Calpain 1) ELISA Kit

The Rat (CAPN1) Calpain 1 ELISA Kit measures Calpain 1 in Rat samples. The plate has been pre-coated with Rat CAPN1 antibody. CAPN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CAPN1 Antibody is added and binds to CAPN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CAPN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CAPN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1748Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.049 ng/mL
Detection Range 0.1-40 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-364', 'Gly-377' and 'His-399'. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Calpain 1
Regulatory Status For Research Use Only
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