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Rat CAPN2 (Calpain-2 Catalytic Subunit) ELISA Kit

Rat CAPN2 (Calpain-2 Catalytic Subunit) ELISA Kit

The Rat (CAPN2) Calpain-2 Catalytic Subunit ELISA Kit measures Calpain-2 Catalytic Subunit in Rat samples. The plate has been pre-coated with Rat CAPN2 antibody. CAPN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CAPN2 Antibody is added and binds to CAPN2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CAPN2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CAPN2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1325Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.021 ng/mL
Detection Range 0.05-30 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Calpain-2 Catalytic Subunit
Regulatory Status For Research Use Only
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