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Rat CHRM2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit

Rat CHRM2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit

The Rat (CHRM2) Muscarinic Acetylcholine Receptor M2 ELISA Kit measures Muscarinic Acetylcholine Receptor M2 in Rat samples. The plate has been pre-coated with Rat CHRM2 antibody. CHRM2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CHRM2 Antibody is added and binds to CHRM2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHRM2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CHRM2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1491Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 3.71 ng/L
Detection Range 6-1800 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium Channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Muscarinic Acetylcholine Receptor M2
Regulatory Status For Research Use Only
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