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Rat CHRM3 (Muscarinic Acetylcholine Receptor M3) ELISA Kit

Rat CHRM3 (Muscarinic Acetylcholine Receptor M3) ELISA Kit

The Rat (CHRM3) Muscarinic Acetylcholine Receptor M3 ELISA Kit measures Muscarinic acetylcholine receptor M3 in Rat samples. The plate has been pre-coated with Rat CHRM3 antibody. CHRM3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CHRM3 Antibody is added and binds to CHRM3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHRM3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CHRM3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2158Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.033 ng/mL
Detection Range 0.06-18 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Neuroscience
Background The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Muscarinic acetylcholine receptor M3
Regulatory Status For Research Use Only
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