The Rat (CYP11A1) Cholesterol Side-chain Cleavage Enzyme, Mitochondrial ELISA Kit measures Cholesterol side-chain cleavage enzyme, mitochondrial in Rat samples. The plate has been pre-coated with Rat CYP11A1 antibody. CYP11A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CYP11A1 Antibody is added and binds to CYP11A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP11A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CYP11A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasCardiovascular, Enzyme & Kinase, Lipid and Metabolism, Metabolism
BackgroundA cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Source: UniProt Consortium (2025)
