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Rat CYP11B2 (Cytochrome P450 11B2, Mitochondrial) ELISA Kit
Rat CYP11B2 (Cytochrome P450 11B2, Mitochondrial) ELISA Kit
The Rat (CYP11B2) Cytochrome P450 11B2, Mitochondrial ELISA Kit measures Cytochrome P450 11B2, mitochondrial in Rat samples. The plate has been pre-coated with Rat CYP11B2 antibody. CYP11B2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CYP11B2 Antibody is added and binds to CYP11B2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP11B2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CYP11B2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1940Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
9.73 ng/L
Detection Range
20-6000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
A cytochrome P450 monooxygenase that catalyzes the biosynthesis of adrenal mineralocorticoid aldosterone. Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone/21-hydroxyprogesterone, namely 11-beta hydroxylation followed with two successive oxidations at C18 to yield 18-hydroxy and then 18-aldehyde derivatives, resulting in the formation of aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Cytochrome P450 11B2, mitochondrial
Regulatory Status
For Research Use Only
