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Rat CYP51A1 (Lanosterol 14 Alpha Demethylase) ELISA Kit
Rat CYP51A1 (Lanosterol 14 Alpha Demethylase) ELISA Kit
The Rat (CYP51A1) Lanosterol 14 Alpha Demethylase ELISA Kit measures Lanosterol 14 alpha demethylase in Rat samples. The plate has been pre-coated with Rat Cyp51a1 antibody. Cyp51a1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Cyp51a1 Antibody is added and binds to Cyp51a1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Cyp51a1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat Cyp51a1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1518Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
0.026 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid (PubMed:7665087, PubMed:10544287, PubMed:11328599).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:7665087, PubMed:10544287, PubMed:11328599). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Lanosterol 14 alpha demethylase
Regulatory Status
For Research Use Only
