The Rat (EPI) Epinephrine ELISA Kit measures EpinephrineAdrenaline in Rat samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of EPI in the sample. The concentration of EPI in the sample is then determined by comparing the O.D. of the samples to the standard curve.
Research AreasNeuroscience, Nephrology, Kidney biomarker
BackgroundAdrenaline, also known as epinephrine, is a hormone and medication which is involved in regulating visceral functions (e.g., respiration). Adrenaline is normally produced both by the adrenal glands and by a small number of neurons in the medulla oblongata. It plays an important role in the fight-or-flight response by increasing blood flow to muscles, output of the heart by acting on SA Node, pupil dilation response and blood sugar level. It does this by binding to alpha and beta receptors. It is found in many animals and some single-celled organisms. Polish physiologist Napoleon Cybulski first isolated adrenaline in 1895. Source: UniProt Consortium (2025)
