1
/
of
1
Rat ERBB2 (Receptor Tyrosine-protein Kinase Erbb-2) ELISA Kit
Rat ERBB2 (Receptor Tyrosine-protein Kinase Erbb-2) ELISA Kit
The Rat (ERBB2) Receptor Tyrosine-protein Kinase Erbb-2 ELISA Kit measures Receptor tyrosine-protein kinase erbB-2 in Rat samples. The plate has been pre-coated with Rat ERBB2 antibody. ERBB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ERBB2 Antibody is added and binds to ERBB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ERBB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ERBB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2460Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Rat
Sensitivity
0.29 ng/mL
Detection Range
0.5-100 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Tags and Cell Markers, Signal Transduction, Cancer, Enzyme & Kinase
Background
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).
Interacts (preferentially with the tyrosine phosphorylated form) with CPNE3; this interaction occurs at the cell membrane and is increased in a growth factor heregulin-dependent manner (By similarity).
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Receptor tyrosine-protein kinase erbB-2
Regulatory Status
For Research Use Only
