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Rat ET-1 (Endothelin 1) ELISA Kit

Rat ET-1 (Endothelin 1) ELISA Kit

The Rat (ET-1) Endothelin 1 ELISA Kit measures Endothelin 1 in Rat samples. The plate has been pre-coated with Rat EDN1 antibody. EDN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat EDN1 Antibody is added and binds to EDN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EDN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat EDN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0462Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 1.02 ng/L
Detection Range 2-600 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cancer
Background Endothelins are endothelium-derived vasoconstrictor Peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Endothelin 1
Regulatory Status For Research Use Only
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