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Rat ET-2 (Endothelin 2) ELISA Kit
Rat ET-2 (Endothelin 2) ELISA Kit
The Rat (ET-2) Endothelin 2 ELISA Kit measures Endothelin 2 in Rat samples. The plate has been pre-coated with Rat ET-2 antibody. ET-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ET-2 Antibody is added and binds to ET-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ET-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ET-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1006Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
0.51 ng/L
Detection Range
1-300 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
Endothelin 2 (ET-2) is a protein encoded by the EDN2 gene in humans. It was first discovered in 1988 by Yanagisawa and team and belongs to a family of three endothelin peptide isoforms (ET-1, ET-2, ET-3), which constrict blood vessels. ET-2 is encoded by genes on separate chromosomes to its isoforms and is mainly produced in vascular endothelial cells of the kidney, placenta, uterus, heart, central nervous system and intestine. It becomes present in the blood of animals and humans at levels ranging from 0.3pg/ml to 3pg/ml. ET-2 acts by binding to two different G-protein coupled receptors (GCPRs), the endothelin A receptor (EDNRA) and the endothelin B receptor (EDNRB). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Endothelin 2
Regulatory Status
For Research Use Only
