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Rat F2 (Prothrombin) ELISA Kit

Rat F2 (Prothrombin) ELISA Kit

The Rat (F2) Prothrombin ELISA Kit measures Prothrombin in Rat samples. The plate has been pre-coated with Rat F2 antibody. F2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat F2 Antibody is added and binds to F2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated F2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat F2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2227Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 9.03 ng/mL
Detection Range 20-6000 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Prothrombin
Regulatory Status For Research Use Only
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