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Rat GFER (Fad-linked Sulfhydryloxidase Alr) ELISA Kit
Rat GFER (Fad-linked Sulfhydryloxidase Alr) ELISA Kit
The Rat (GFER) Fad-linked Sulfhydryloxidase Alr ELISA Kit measures FAD-linked sulfhydryl oxidase ALR in Rat samples. The plate has been pre-coated with Rat GFER antibody. GFER present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GFER Antibody is added and binds to GFER in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GFER antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GFER. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2486Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
11.57 ng/L
Detection Range
20-4500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
FAD-linked sulfhydryl oxidase ALR
Regulatory Status
For Research Use Only
