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Rat GPX-4 (Glutathione Peroxidase 4) ELISA Kit
Rat GPX-4 (Glutathione Peroxidase 4) ELISA Kit
The Rat (GPX-4) Glutathione Peroxidase 4 ELISA Kit measures Glutathione peroxidase 4 in Rat samples. The plate has been pre-coated with Rat GPx-4 antibody. GPx-4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GPx-4 Antibody is added and binds to GPx-4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GPx-4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GPx-4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1787Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
0.31 mU/L
Detection Range
0.5-300 mU/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Oxidative Stress
Background
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (By similarity).
Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (By similarity).
Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (PubMed:1556123, PubMed:9988735).
Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species (By similarity).
The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (By similarity).
The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (By similarity).
May be required to protect cells from the toxicity of ingested lipid hydroperoxides (By similarity).
Required for normal sperm development and male fertility (By similarity).
Essential for maturation and survival of photoreceptor cells (By similarity).
Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity).
Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism (By similarity).
Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway (By similarity).
Specifically able to suppress the production of leukotriene and prostaglandin in response to several stimuli by reducing fatty acid hydroperoxide (PubMed:9442035, PubMed:11010961).
Specifically required to prevent mitochondrial cell death by mediating reduction of cardiolipin hydroperoxide (PubMed:9988735, PubMed:10506188, PubMed:10998361).
Also required for normal sperm development and male fertility (By similarity).
Required for male fertility by stabilizing the condensed chromatin in sperm nuclei. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Glutathione peroxidase 4
Regulatory Status
For Research Use Only
