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Rat HPT (Haptoglobin) ELISA Kit

Rat HPT (Haptoglobin) ELISA Kit

The Rat (HPT) Haptoglobin ELISA Kit measures Haptoglobin in Rat samples. The plate has been pre-coated with Rat HP antibody. HP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HP Antibody is added and binds to HP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0357Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.022 ug/mL
Detection Range 0.05-20 ug/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cardiovascular
Background As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Haptoglobin
Regulatory Status For Research Use Only
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