Rat HSD11Beta1 (11-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit
Rat HSD11Beta1 (11-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit
The Rat HSD11Beta1 (11-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSD11Beta1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSD11Beta1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSD11Beta1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSD11Beta1. You can calculate the concentration of Rat HSD11Beta1 in the samples by comparing the OD of the samples to the standard curve.
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