The Rat (HSD3B1) 3 Beta-Hydroxysteroid Dehydrogenase, Delta 5--＞4-isomerase Type 1 ELISA Kit measures 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 in Rat samples. The plate has been pre-coated with Rat HSD3B1 antibody. HSD3B1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSD3B1 Antibody is added and binds to HSD3B1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSD3B1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HSD3B1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasSignal Transduction, Cancer, Metabolism
BackgroundA bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta5-steroid precursors to 3-oxo-Delta4-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone (PubMed:1537836, PubMed:1985917).
Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1537836).
Expected to use NAD+ as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
