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Rat HSP10 (10 kDa Heat Shock Protein, Mitochondrial) ELISA Kit
Rat HSP10 (10 kDa Heat Shock Protein, Mitochondrial) ELISA Kit
The Rat (HSP10) 10 kDa Heat Shock Protein, Mitochondrial ELISA Kit measures 10 kDa heat shock protein, mitochondrial in Rat samples. The plate has been pre-coated with Rat HSP10 antibody. HSP10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSP10 Antibody is added and binds to HSP10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSP10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HSP10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2513Ra
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Rat
Sensitivity
0.33 ng/mL
Detection Range
0.5-100 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
10 kDa heat shock protein, mitochondrial
Regulatory Status
For Research Use Only
