• Rat (IgM) Immunoglobulin M ELISA Kit
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Rat IgM (Immunoglobulin M) ELISA Kit

Rat IgM (Immunoglobulin M) ELISA Kit

The Rat (IgM) Immunoglobulin M ELISA Kit measures Immunoglobulin M in Rat samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of IgM in the sample. The concentration of IgM in the sample is then determined by comparing the O.D. of the samples to the standard curve.

Catalog No: EA0034Ra
$595.00 USD
$458.00 USD $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 8.15 ng/mL
Detection Range 15 - 3000 ng/mL
Sample Type Serum, plasma, cell culture supernatant
Incubation(s) 2.5 hour(s)
Research Areas Immunology
Background Immunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. In the case of humans and other mammals that have been studied, the spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Immunoglobulin M
Regulatory Status For Research Use Only
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