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Rat Interleukin-1 Receptor Type 2 (IL-1r2) ELISA Kit

Rat Interleukin-1 Receptor Type 2 (IL-1r2) ELISA Kit

This ELISA kit is designed to detect Rat Interleukin-1 Receptor Type 2 (Rat IL-1r2). The assay plate has been pre-coated with mouse anti-Rat IL1R2 monoclonal antibody. When the sample containing IL1R2 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Rat IL1R2 Antibody is added to the wells and binds to IL1R2 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Rat IL1R2 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE356
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Rat
Sensitivity 5.12 pg/mL
Detection Range 12.5-800 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cardiovascular, Immunology, Stem cells
Background Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Interleukin-1 receptor type 2
Regulatory Status For Research Use Only
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