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Rat LDL (Low Density Lipoprotein) ELISA Kit

Rat LDL (Low Density Lipoprotein) ELISA Kit

The Rat (LDL) Low Density Lipoprotein ELISA Kit measures low density lipoprotein in Rat samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of LDL in the sample. The concentration of LDL in the sample is then determined by comparing the O.D. of the samples to the standard curve.

Catalog No: EA0021Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.052ug/ml
Detection Range 0.1 - 40 ug/mL
Sample Type Serum, plasma, cell culture supernatant
Incubation(s) 2.5 hour(s)
Research Areas Cardiovascular
Background Low-density lipoprotein (LDL) is one of the five major groups of lipoprotein which transport all fat molecules around the body in the extracellular water. These groups, from least dense to most dense, are chylomicrons (aka ULDL by the overall density naming convention), very low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein and high-density lipoprotein (HDL). LDL delivers fat molecules to cells. LDL is involved in atherosclerosis, a process in which it is oxidized within the walls of arteries. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte low density lipoprotein
Regulatory Status For Research Use Only
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