Rat MC3R (Melanocortin Receptor 3) ELISA Kit
Rat MC3R (Melanocortin Receptor 3) ELISA Kit
The Rat MC3R (Melanocortin Receptor 3) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MC3R. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MC3R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MC3R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MC3R. You can calculate the concentration of Rat MC3R in the samples by comparing the OD of the samples to the standard curve.
Couldn't load pickup availability
Product Details
