Rat MIP-1B (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
Rat MIP-1B (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
The Rat (MIP-1B) Macrophage Inflammatory Protein 1 Beta ELISA Kit measures macrophage inflammatory protein 1beta in Rat samples. The plate has been pre-coated with Rat MIP-1beta antibody. MIP- 1beta present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MIP-1beta Antibody is added and binds to MIP-1beta in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MIP-1beta antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MIP-1beta. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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