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Rat MMP-2 (Matrix Metalloproteinase 2) ELISA Kit

Rat MMP-2 (Matrix Metalloproteinase 2) ELISA Kit

The Rat (MMP-2) Matrix Metalloproteinase 2 ELISA Kit measures Matrix Metalloproteinase 2 in Rat samples. The plate has been pre-coated with Rat MMP2 antibody. MMP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MMP2 Antibody is added and binds to MMP2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MMP2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MMP2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0315Ra
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Rat
Sensitivity 0.092 ng/mL
Detection Range 0.2-60 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cancer
Background Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity). PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta3 on the surface of blood vessels (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Matrix Metalloproteinase 2
Regulatory Status For Research Use Only
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